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mouse b6 129s6 chat tm2 cre lowl j  (Jackson Laboratory)

 
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    Jackson Laboratory mouse b6 129s6 chat tm2 cre lowl j
    Mouse B6 129s6 Chat Tm2 Cre Lowl J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) <t>in</t> <t>ChAT‐Cre</t> mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.
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    Image Search Results


    Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) in ChAT‐Cre mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Whole‐Brain Neural Connectivity to Cholinergic Neurons in the Lower Thoracic Intermediolateral Column

    doi: 10.1002/cns.70902

    Figure Lengend Snippet: Experimental strategies for tracing monosynaptic inputs to lower thoracic IML cholinergic neurons. (A) Schematic of experimental design. (B) Schematic of the starter cells in the IML and their presynaptic inputs. (C, D) Representative images showing starter cells (yellow) in ChAT‐Cre mice (C), but no labeled neurons in wild‐type mice (D). Scale bars, 200 μm (main image) and 50 μm (inset). (E) Example fluorescence images show starter cells (marked with white arrowheads), which colocalize mCherry, EGFP and ChAT immunoreactive signals in the IML. Scale bar, 40 μm.

    Article Snippet: Five adult ChAT‐Cre mice (male and female, RRID: IMSR_JAX: 018957, C57BL/6J background strain, 2–4 months, 25–27 g) and three wild‐type littermate mice were used in experiments.

    Techniques: Labeling, Fluorescence